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cdc25  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology cdc25
    Cdc25, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdc25/Cdc25A+Antibody/pm39903385-82-12-13
    Average 94 stars, based on 578 article reviews
    cdc25 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Control:

    Article Title: Comparative effects of arsenic trioxide and chemotherapy on Chk1 and CDC25 gene expression in gastric cancer cells AGS and MKN45: a potential therapeutic strategy.
    Article Snippet: 1 Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran 2 Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran 3 Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran, Iran Abstract Background Gastric cancer (GC) remains a significant global health burden, particularly in East Asia, where it is a leading cause of cancer-related morbidity and mortality.. Despite advancements in chemotherapy, the development of chemoresistance continues to undermine the efficacy of standard treatments such as Docetaxel and Oxaliplatin.. Arsenic trioxide (ATO) has emerged as a potential therapeutic agent capable of overcoming resistance by targeting DNA repair mechanisms, particularly through the downregulation of Checkpoint Kinase 1 (Chk1).

    Incubation:

    Article Title: Comparative effects of arsenic trioxide and chemotherapy on Chk1 and CDC25 gene expression in gastric cancer cells AGS and MKN45: a potential therapeutic strategy.
    Article Snippet: 1 Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran 2 Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran 3 Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Tehran, Iran Abstract Background Gastric cancer (GC) remains a significant global health burden, particularly in East Asia, where it is a leading cause of cancer-related morbidity and mortality.. Despite advancements in chemotherapy, the development of chemoresistance continues to undermine the efficacy of standard treatments such as Docetaxel and Oxaliplatin.. Arsenic trioxide (ATO) has emerged as a potential therapeutic agent capable of overcoming resistance by targeting DNA repair mechanisms, particularly through the downregulation of Checkpoint Kinase 1 (Chk1).

    other:

    Article Title: Escape from Therapy-Induced Accelerated Cellular Senescence in p53-Null Lung Cancer Cells and in Human Lung Cancers
    Article Snippet: Accelerated cellular senescence (ACS) has been described for tumor cells treated with chemotherapy and radiation.. Following exposure to genotoxins, tumor cells undergo terminal growth arrest and adopt morphologic and marker features suggestive of cellular senescence.. ACS is elicited by a variety of chemotherapeutic agents in the p53-null, p16-deficient human non–small cell H1299 carcinoma cells.

    Liposomes:

    Article Title: BECN1 promotes radiation-induced G2/M arrest through regulation CDK1 activity: a potential role for autophagy in G2/M checkpoint
    Article Snippet: .. The reagents used in this study were as follows: liposomes were purchased from Invitrogen (Carlsbad, CA, USA); glycine, lauryl sodium sulfate, tetramethylethylenediamine, TRIzol, and tris(hydroxymethyl)aminomethane were purchased from Amresco (Solon, OH, USA); acrylic amide was purchased from Merck (Darmstadt, Germany); bovine serum albumin (BSA) was purchased from Roche (Basel, Switzerland); fluorescent protein solutions were purchased from Pierce (Rockford, IL, USA); ammonium peroxydisulfate, dimethyl sulfoxide (DMSO), N , N’ -methylenebisacrylamide, and puromycin were purchased from Sigma (St. Louis, MO, USA); trypsin, Dulbecco’s modified Eagle’s medium (DMEM) with high glucose, and fetal bovine serum were purchased from HyClone (Logan, UT, USA); PrimeSTAR DNA polymerase and T4 DNA ligase were purchased from TaKaRa (Tokyo, Japan); GAPDH, P62, CHK1, CHK2, MYTl, WEEl, CDC25, CDC25C, ATM, CDK1, LC3, p68-CHK1, p216-CDC25C, p15-CDK1, p1981-ATM, SQSTM1/P62, pCHK2, pCHK1, CDC, pCDC25C, and MPM2 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), Cell Signaling Technology (Danvers, MA, USA), or Millipore/Upstate(NY, USA); and the pLKO.1 plasmid was purchased from Sigma (Darmstadt, Germany). .. Human A549 cells and the human TNBC MDA-MB-231 cell line were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Modification:

    Article Title: BECN1 promotes radiation-induced G2/M arrest through regulation CDK1 activity: a potential role for autophagy in G2/M checkpoint
    Article Snippet: .. The reagents used in this study were as follows: liposomes were purchased from Invitrogen (Carlsbad, CA, USA); glycine, lauryl sodium sulfate, tetramethylethylenediamine, TRIzol, and tris(hydroxymethyl)aminomethane were purchased from Amresco (Solon, OH, USA); acrylic amide was purchased from Merck (Darmstadt, Germany); bovine serum albumin (BSA) was purchased from Roche (Basel, Switzerland); fluorescent protein solutions were purchased from Pierce (Rockford, IL, USA); ammonium peroxydisulfate, dimethyl sulfoxide (DMSO), N , N’ -methylenebisacrylamide, and puromycin were purchased from Sigma (St. Louis, MO, USA); trypsin, Dulbecco’s modified Eagle’s medium (DMEM) with high glucose, and fetal bovine serum were purchased from HyClone (Logan, UT, USA); PrimeSTAR DNA polymerase and T4 DNA ligase were purchased from TaKaRa (Tokyo, Japan); GAPDH, P62, CHK1, CHK2, MYTl, WEEl, CDC25, CDC25C, ATM, CDK1, LC3, p68-CHK1, p216-CDC25C, p15-CDK1, p1981-ATM, SQSTM1/P62, pCHK2, pCHK1, CDC, pCDC25C, and MPM2 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), Cell Signaling Technology (Danvers, MA, USA), or Millipore/Upstate(NY, USA); and the pLKO.1 plasmid was purchased from Sigma (Darmstadt, Germany). .. Human A549 cells and the human TNBC MDA-MB-231 cell line were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Plasmid Preparation:

    Article Title: BECN1 promotes radiation-induced G2/M arrest through regulation CDK1 activity: a potential role for autophagy in G2/M checkpoint
    Article Snippet: .. The reagents used in this study were as follows: liposomes were purchased from Invitrogen (Carlsbad, CA, USA); glycine, lauryl sodium sulfate, tetramethylethylenediamine, TRIzol, and tris(hydroxymethyl)aminomethane were purchased from Amresco (Solon, OH, USA); acrylic amide was purchased from Merck (Darmstadt, Germany); bovine serum albumin (BSA) was purchased from Roche (Basel, Switzerland); fluorescent protein solutions were purchased from Pierce (Rockford, IL, USA); ammonium peroxydisulfate, dimethyl sulfoxide (DMSO), N , N’ -methylenebisacrylamide, and puromycin were purchased from Sigma (St. Louis, MO, USA); trypsin, Dulbecco’s modified Eagle’s medium (DMEM) with high glucose, and fetal bovine serum were purchased from HyClone (Logan, UT, USA); PrimeSTAR DNA polymerase and T4 DNA ligase were purchased from TaKaRa (Tokyo, Japan); GAPDH, P62, CHK1, CHK2, MYTl, WEEl, CDC25, CDC25C, ATM, CDK1, LC3, p68-CHK1, p216-CDC25C, p15-CDK1, p1981-ATM, SQSTM1/P62, pCHK2, pCHK1, CDC, pCDC25C, and MPM2 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), Cell Signaling Technology (Danvers, MA, USA), or Millipore/Upstate(NY, USA); and the pLKO.1 plasmid was purchased from Sigma (Darmstadt, Germany). .. Human A549 cells and the human TNBC MDA-MB-231 cell line were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).



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    Primer sequences for qRT-PCR

    Journal: Biomolecules and Biomedicine

    Article Title: F13B regulates angiogenesis and tumor progression in hepatocellular carcinoma via the HIF-1α/VEGF pathway

    doi: 10.17305/bb.2024.10794

    Figure Lengend Snippet: Primer sequences for qRT-PCR

    Article Snippet: The primary antibodies used were F13B (cat No: 20269-1-AP), CD31 (cat No: 11265-1-AP), p-VEGFR2/VEGFR2 (cat No: 26415-1-AP), MMP2 (cat No: 10373-2-AP), MMP9 (cat No: 10375-2-AP), p-AKT/AKT (cat No: 28731-1-AP / 10176-2-AP), VEGF (cat No: 66828-1-Ig), Cyclin B1 (cat No: 55004-1-AP), CDC2 (cat No: 15646-1-AP), CDC25 (cat No: 55031-1-AP), p21 (cat No: 10355-1-AP) (1:1000), and Cyclin D1 (cat No: 60186-1-Ig), VEGFA (cat No: 19003-1-AP), p-mTOR/mTOR (cat No: 67778-1-Ig / 66888-1-Ig), HIF-1α (cat No: 20960-1-AP) (1:5000) (Wuhan Sanying Biotechnology, Wuhan, China).

    Techniques: Sequencing

    Effects of knockdown or overexpression of F13B on cyclin expression in HCC. (A–F) After F13B overexpression, changes in cyclin expression (Cyclin B1, CDC2, CDC25, p21, and Cyclin D1) in HCC cells were measured via qRT-PCR and WB; (G–L) After F13B knockdown, changes in cyclin expression in HCC cells were measured via qRT-PCR and WB. * P < 0.05. HCC: Hepatocellular carcinoma; qRT-PCR: Quantitative real-time polymerase chain reaction; WB: Western blotting.

    Journal: Biomolecules and Biomedicine

    Article Title: F13B regulates angiogenesis and tumor progression in hepatocellular carcinoma via the HIF-1α/VEGF pathway

    doi: 10.17305/bb.2024.10794

    Figure Lengend Snippet: Effects of knockdown or overexpression of F13B on cyclin expression in HCC. (A–F) After F13B overexpression, changes in cyclin expression (Cyclin B1, CDC2, CDC25, p21, and Cyclin D1) in HCC cells were measured via qRT-PCR and WB; (G–L) After F13B knockdown, changes in cyclin expression in HCC cells were measured via qRT-PCR and WB. * P < 0.05. HCC: Hepatocellular carcinoma; qRT-PCR: Quantitative real-time polymerase chain reaction; WB: Western blotting.

    Article Snippet: The primary antibodies used were F13B (cat No: 20269-1-AP), CD31 (cat No: 11265-1-AP), p-VEGFR2/VEGFR2 (cat No: 26415-1-AP), MMP2 (cat No: 10373-2-AP), MMP9 (cat No: 10375-2-AP), p-AKT/AKT (cat No: 28731-1-AP / 10176-2-AP), VEGF (cat No: 66828-1-Ig), Cyclin B1 (cat No: 55004-1-AP), CDC2 (cat No: 15646-1-AP), CDC25 (cat No: 55031-1-AP), p21 (cat No: 10355-1-AP) (1:1000), and Cyclin D1 (cat No: 60186-1-Ig), VEGFA (cat No: 19003-1-AP), p-mTOR/mTOR (cat No: 67778-1-Ig / 66888-1-Ig), HIF-1α (cat No: 20960-1-AP) (1:5000) (Wuhan Sanying Biotechnology, Wuhan, China).

    Techniques: Knockdown, Over Expression, Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot